LaserToF LT2 PlusBenchtop MALDI Time of Flight MS SAI's LaserToF LT2 Plus is a completely automated, compact and versatile matrix assisted laser desorption ionisation time of flight (MALDI-TOF) mass analyser. The instrument is designed for the rapid and accurate analysis of a wide range of biomolecules such as peptides, proteins and oligonucleotides. The LT2 Plus is also suitable for the analysis of industrial polymers as used in the petroleum and chemical additive industries.
Key Features - Linear MALDI-ToF - 96 well 1/4 Microtitre plate - Pulsed Extraction - Simple operation - Sample viewing camera - Small Footprint Mass Spectrometer - Advanced acquisition and control electronics - High stability and easy calibration for optimum precision for a wide variety of measurements. - Fast electron multiplier detector system as standard for linear operation - High gain, large dynamic and linear range to improve the system performance - Detector has long lifetime and low maintenance Software - Full control of all MS parameters - Data acquisition and data reduction program - Automated sequence programming for batch operation - Database search of peptide map data - The system comes network ready |
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LaserToF LT3 PlusBenchtop MALDI-ToF-TOF MS-MS SAI's LaserToF LT3 Plus is a completely automated, compact and versatile matrix assisted laser desorption ionisation time of flight (MALDI-TOF) mass analyser. The instrument is designed for the rapid and accurate analysis of a wide range of biomolecules such as peptides, proteins and oligonucleotides. The LT3 Plus is also suitable for the analysis of industrial polymers as used in the petroleum and chemical additive industries.
Key Features - Reflectron & Linear MALDI-ToF - 96 well 1/4 Microtitre plate - Pulsed Extraction - Simple operation - Sample viewing camera - Small Footprint Mass Spectrometer - Advanced acquisition and control electronics - High stability and easy calibration for optimum precision for a wide variety of measurements - Fast electron multiplier detector system as standard for linear operation - Fast microchannel plate detector system as standard for reflectron operation - High gain, large dynamic and linear range to improve the system performance - Detectors have long lifetime and low maintenance Software - Full control of all MS parameters - Data acquisition and data reduction program - Automated sequence programming for batch operation - Database search of peptide map data - The system comes network ready |
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LaserToF TTLaserToF TT is a Research Grade MALDI-MS/ MS instrument, incorporating some of the most advanced performance and design features of any mass spectrometer. The TT is the result of an accumulated knowledge base. It offers rapid and accurate single scan seamless MS/MS analysis. The design combines high resolution peptide mass fingerprinting and peptide sequencing by uni or bi molecular fragmentation. The instrument is equipped with a 2D sample stage (including 96/384/1536-well format) for high throughput automated analysis.
Key Features - Attomole Sensitivity - Novel "ZOOM" Lens - Full Imaging Capability - Collision Cell (CID) - Post Source Decay (PSD) - Ideal field Reflectron - Full Proteomics Software - Nd:YAG Laser for high repetition rates Mass Spectrometer - Dual Time of Flight Mass Analysers : linear and ideal reflectron - Novel Ideal Field reflectron design enabling seamless MS/MS in a single scan - Unique 2-D sample stage with fast change over and precise positioning - Integrated sample viewing camera and laser optics to give maximum control of experiments - Advanced acquisition and control electronics capable of operation with high repetition laser frequencies - High stability and automatic calibration for maximum precision in peptide measurements Detectors - Long lifetime low maintenance fast electron multiplier detection system as standard for linear operation - Fast hybrid multiplier detector as standard for reflectron operation giving high gain, large dynamic and linear range for improved system performance Software - Full control of all MS parameters - Data acquisition and data reduction program - Automated sequence programming for batch operation - Integrated camera and viewing software - Report generator - Database search of both peptide map and PSD data - The system comes network ready |
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Life SCIENCE
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The two most common techniques in the bottom up approach to analytical proteomics are equally well served by SAI’s powerful range of MALDI TOF and MALDI TOF-TOF instruments
Peptide mass fingerprinting (peptide mapping) Peptide mass fingerprinting is a protein identification technique in which MALDI MS can be used to measure the masses of proteolytic peptide fragments. The protein is then identified by matching the measured masses to corresponding peptide masses from protein or nucleotide sequence databases. Peptide mass fingerprinting works well for analytical proteomics because it combines a conceptually simple approach with robust, high throughput instrumentation such as SAI's LaserToF MALDI range. As with other MS based analytical proteomics techniques, the quality of the protein identifications depends on the quality of the MS data itself, the accuracy of the databases, and the power of the search algorithms and software used. Fortunately for the user, SAI can offer all three of the ingredients for success in this approach to proteomics and we have a range of application notes to demonstrate this. Please click on items of interest on the right to find out more or download documents. Tandem MS In MS-MS mode, a peptide ion is isolated in the mass analyzer and subjected to downstream dissociation to produce product ion fragments. The amino acid sequence of the original precursor ion can be deduced from the masses of the fragment ions; this forms the basis for de novo sequencing by MS-MS. Fragmentation data can be used to determine a short stretch of amino acid sequence (a "sequence tag"), which can be used to search a database. A more convenient approach is to compare product ion spectra directly with databases to identify the intact protein. SAI’s linear-reflectron ToF-ToF mass spectrometers are specifically designed to offer both post source decay (PSD) and collision induced dissociation (CID) methods achieving accurate seamless single scan MS-MS data in the most efficient way possible. |
Polymers
One of the key advantages of MALDI MS for synthetic polymer analysis is that the absolute molecular weights of oligomers can be determined as opposed to obtaining relative molecular weights by chromatographic techniques.
MALDI polymer analysis does not require polymer standards to assign molecular weights to oligomers, and the technique permits accurate determination of molecular weights from narrowly distributed polymers (polydispersity <1.2).
Using submilligram amounts of sample material, the actual analysis can be accomplished in a few minutes. Therefore, the speed and information obtained by MALDI are significantly greater than with other conventional molecular weight determination techniques. In addition, MALDI can determine molecular weight independent of polymer structure.
For example, rigid-rod polymers such as tetrahydropyrene are a challenge to analyze by conventional polymer analysis methods such as gel permeation chromatography (GPC) because these polymers have a linear stick like geometry.
Most synthetic polymers have structures with flexible backbones that form random coil conformations in solution.
These polymers are readily analyzed by GPC methods, which are based on correlating the hydrodynamic volume of the coiled polymer chains with molecular weight.
However, the hydrodynamic volume of rigid-rod polymers does not correlate with polymer molecular weight; hence, molecular weights determined by GPC analysis of these structures often deviate strongly from the true values. Thus MALDI MS can be used to analyze materials that are difficult to characterize by GPC methods, because of either solvent or column incompatibilities.
In addition, GPC does not perform well in low-molecular-weight regions (masses less than a few kilodaltons), whereas MALDI works very effectively in this mass range.
Polymer characteristics that can readily be determined by MALDI MS include molecular weight averages (including the number [Mn] and weight [Mw] averaged molecular weights), polydispersity, mass of repeat units, and end group mass structure. Molecular weight and polydispersity data can be used to verify synthetic pathways, study degradation mechanisms, look for additives and impurities, compare product formulations, and provide QC data on batch-to-batch compositional variations.
End-group and chemical structure data are critical to understanding structure-property relationships of polymer formulations.
MALDI MS provides a direct method of determining end-group mass and composition as well as the mass and composition of oligomer repeat units. The successful characterization of synthetic polymers by MALDI MS is strongly dependent on the chemical structure of the polymer.
It is important to point out that not all synthetic polymers are readily analyzed by MALDI MS, and polymers with different chemical structures may require different sample preparation for a successful analysis. From a practical standpoint, analyzable polymers can be classified into four groups : water-soluble polymers, such as poly(acrylic acid) and poly(ethylene glycol) (PEG); polar organic-soluble polymers, such as acrylics and poly(methyl methacrylate) (PMMA); non polar organic-soluble polymers, such as polystyrene (PS), polyvinyl chloride,and polyethylene; and low-solubility polymers, such as cured polyimide. This last class includes polymers soluble in solvents that are not compatible with matrix materials, and these are the most difficult to analyze by MALDI because a homogeneous mixture of matrix and analyte is not easily prepared.
MALDI polymer analysis does not require polymer standards to assign molecular weights to oligomers, and the technique permits accurate determination of molecular weights from narrowly distributed polymers (polydispersity <1.2).
Using submilligram amounts of sample material, the actual analysis can be accomplished in a few minutes. Therefore, the speed and information obtained by MALDI are significantly greater than with other conventional molecular weight determination techniques. In addition, MALDI can determine molecular weight independent of polymer structure.
For example, rigid-rod polymers such as tetrahydropyrene are a challenge to analyze by conventional polymer analysis methods such as gel permeation chromatography (GPC) because these polymers have a linear stick like geometry.
Most synthetic polymers have structures with flexible backbones that form random coil conformations in solution.
These polymers are readily analyzed by GPC methods, which are based on correlating the hydrodynamic volume of the coiled polymer chains with molecular weight.
However, the hydrodynamic volume of rigid-rod polymers does not correlate with polymer molecular weight; hence, molecular weights determined by GPC analysis of these structures often deviate strongly from the true values. Thus MALDI MS can be used to analyze materials that are difficult to characterize by GPC methods, because of either solvent or column incompatibilities.
In addition, GPC does not perform well in low-molecular-weight regions (masses less than a few kilodaltons), whereas MALDI works very effectively in this mass range.
Polymer characteristics that can readily be determined by MALDI MS include molecular weight averages (including the number [Mn] and weight [Mw] averaged molecular weights), polydispersity, mass of repeat units, and end group mass structure. Molecular weight and polydispersity data can be used to verify synthetic pathways, study degradation mechanisms, look for additives and impurities, compare product formulations, and provide QC data on batch-to-batch compositional variations.
End-group and chemical structure data are critical to understanding structure-property relationships of polymer formulations.
MALDI MS provides a direct method of determining end-group mass and composition as well as the mass and composition of oligomer repeat units. The successful characterization of synthetic polymers by MALDI MS is strongly dependent on the chemical structure of the polymer.
It is important to point out that not all synthetic polymers are readily analyzed by MALDI MS, and polymers with different chemical structures may require different sample preparation for a successful analysis. From a practical standpoint, analyzable polymers can be classified into four groups : water-soluble polymers, such as poly(acrylic acid) and poly(ethylene glycol) (PEG); polar organic-soluble polymers, such as acrylics and poly(methyl methacrylate) (PMMA); non polar organic-soluble polymers, such as polystyrene (PS), polyvinyl chloride,and polyethylene; and low-solubility polymers, such as cured polyimide. This last class includes polymers soluble in solvents that are not compatible with matrix materials, and these are the most difficult to analyze by MALDI because a homogeneous mixture of matrix and analyte is not easily prepared.