• Bacterion Sytem
  • BactoSCREEN software
  • How it Works
  • Accessories & Consumables
  • Applications
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Bacterial Screening

The Maldi Bacterion is a microbiological analysis system, which consists of SAI’s powerful and compact LaserToF 2 Plus tabletop MALDI-TOF mass spectrometer and their powerful BactoSCREEN-ID software package for device control, spectral analysis and identification of microorganisms including bacteria, Mycobacteria ,yeasts and fungi. It is intended for use for clinical and research diagnostics in a large variety of settings.
​The system is designed for specific identification of microorganisms, testing their antimicrobial susceptibility and identifying genetic determinants of drug resistance by means of  MALDI-TOF.
​BactoSCREEN-ID software is designed to rapidly identify the taxonomy of a microorganism under study at the species level by comparing its mass spectrometry profile with the profiles of known strains of micro- organisms present in the corresponding database.
The software BactoSCREEN have special sections to perform sensitivity studies on antibiotic destruction of bacterial cells, as well as for the detection of resistant strains, which is produced as the detection of bio degradation of antibiotic products, and so the detection of specific genetic markers of resistance).
Applications Include:
  - Clinical diagnostics                 
  - Epidemiological centres                     
  - Food industry                                                
  - Forensic analysis                 
  - Veterinary laboratories                           
  - Research laboratories
  - Medical devices                    
  - Quality control laboratories                   
  - Environmental monitoring                         
  - Pharmaceutical industry       
​  - Vaccine development                   
​  - Microbiological and bacteriological labs                                                                                                                                                                 

BactoSCREEN-ID

The BactoSCREEN-ID software package from SAI are for device control, spectral analysis and identification of microorganisms including bacteria, yeasts Mycobacteria and fungi. It is intended for use for clinical and research diagnostics in a large variety of settings. The system is designed for specific identification of microorganisms, testing their antimicrobial susceptibility and identifying genetic determinants of drug resistance by means of matrix-assisted laser de- sorption ionization time-of-flight mass spectrometry .
BactoSCREEN-ID software is designed to rapidly identify the taxonomy of a microorganism under study at the species level by comparing its mass spectrometry profile with the profiles of known strains of micro- organisms present in the corresponding database.
  • Fast identification of organisms (<25 Min for 96 samples)
  • Multi Status progress monitoring by sample plate well positions
  • Colour Coded spectral matching with database entries
  • Data archival
  • Report generation
  • Confidence scoring system
  • Over 95% accuracy
  • High performance algorithm provides sub 3 second ID performance
  • Novel genomic approaches provide superior biological discrimination
  • Comprehensive database of over 4000 entries
  • Video
  • ​ID Workflow
  • ID Direct from Blood 
  • ID Direct from Urine
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​The identification of microorganisms is possible from solid or liquid medium.
1 - Collection of an isolated colony from the culture medium .
2 - Deposit of this colony on a well on the Sample plate .
3 - Addition of 1 μL of matrix (HCCA                 solution) to each well
4- Insert The Sample plate in The MALDI-TOF
5- The software :
  • Obtain the sample spectrum
  • comparing the unknown spectrum to the spectra of the microorganism from the database;
  • microorganism identification

Rapid Identifcation of Bacteria from Blood Cultures

  • Centrifuge1-2ml in a Vacuette tube with  gel (10min.,3000rpm);
  • remove all of  erythrocytes
  • Re-suspend the sediment in 1ml and transfer in to a 1.5ml Eppendorf tube
  • Centrifuge(1min.,3000rpm)
  • Transfer 1ml of the supernatant in to a new tube and centrifuge(1min,13000rpm)
  • Suspend pelllet in 300µl water
  • Standard extraction protocol for MALDI bacterial profiling
  • Spotting of 1µl extract on to MALDI sample target
  • overlay with HCCA matrix

 Rapid Identification of Bacteria direct from Urine 

  • Urine (1 ml)
  • Centrifuge(30 s, 2000g)
  • Transfer supernatant in new cup
  • Centrifuge (5 min., 15500g)
  • Dry pellet with cotton swab
  • Wash once with de-ionizedwater
  • Standard  extraction protocol for bacterial profiling
  • Spot 1 µl on to MALDI Sample target, overlay withHCCA matrix

Re-usable Sample Plates

The plates of the LT2 allow the deposit of 96 samples. Each well is delimited by a groove encrusted in the plate to facilitate deposition and avoid the risk of contamination. The PLC's plates are reusable thanks to the simple cleaning protocol, which has undergone numerous contamination tests, residual risks and deterioration of the coating .

MALDI-TOF  Kit for 1000 Samples

The kit includes all the reagents necessary for identification of microorganisms by MALDI - TOF mass spectrometry for 1000 Samples:
  • HCCA matrix (4 tubes, 2.5 mg) 
  • bacterial standard,
  • organic solvent (OS, 2ml)
  • 70% formic acid (700 .mu.l),
  • Acetonitrile (600 .mu.l).

Pharma

​•Trace-back of contaminations
•Control of raw materials
•21 CFR Part 11 Support

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Enviromental

​•Ballast water analysis
•Water & Waste Water Analysis
•Taxonomy
•Dereplication of microbial
communities

Food

​•Trace-back of contaminations
•Dairy products
•Beer production
•Rapid Salmonella detection
•Fish and meat species

Animal Health

•Mastitis
•Difficult organisms
•Subtyping to differentiate
dangerous infections from
opportunistic pathogens
•Tracking of microbes specific
for certain poultry flocks.